Figure 4.

Fluorescence microscopy characterization of cellular barriers in MFC. (A) Schematic figure of location of cellular barrier on the interface between gel phase and perfusion channel. (B), (C) Cellular barrier comprised of MDCK_VB6-CB cells. Cells are located on the interface between the perfusion channel and hydrogel as well as on the surface of micro pillars and proliferate to eventually cover the complete surface of the perfusion channel. Cells expressing green fluorescent Vimentin chromobody (VB6-CB B) were fixed with PFA and DAPI staining was performed (C). Cells were seeded at a density of 10⁵ cells per channel at passage 3. Staining was performed at day five after seeding. Single slice confocal microscope image at bottom of the channel. Scale bar 50 µm.