Figure A4.

Success of priming of MFC with the hydrogel solution depends on pillar size and spacing as reflected by a factor α, with α being defined as the product of the pillar width and distance between two adjacent pillars. MFCs were filled at a flow rate of 2.5–3 µL/min with hydrogel solution. Successful priming is achieved when no spillover occurs and perfusion of the adjacent “luminal” channel remains possible.