Table 1.

Reagent volumes for 120 μL gel for one MFC.

3-D Life Reagents	V (μL)	V (μL)
10× CB (80% pH 7.2, 20% pH 5.5)	2.4	9.6
Water	15.3	61.2
SG-PVA (30 mmol/L SH-reactive groups)	2.5	10.0
RGD Peptide (20 mmol/L SH groups)	0.8	3.2
Cell suspension (HT29)	6.0	24.0
CD-Link (20 mmol/L SH groups)	3.0	12.0
Total	30.0	120.0