Figure 2.

Effect of oclacitinib (OCL) on the number of IFN-γ- and IL-4-producing CD8⁺ T cells. The relative (A,C) and absolute count (B,D) of IFN-γ- and IL-4-producing CD8⁺ T cells were determined in cell cultures incubated with or without (control) OCL (10⁻⁶ M and 10⁻⁷ M). The relative count is expressed as a percentage of IFN-γ- or IL-4- producing cells within CD8⁺ T cells. The absolute count represents the number of IL-4⁻IFN-γ⁺CD8⁺ and IL-4⁺IFN-γ⁻CD8⁺ T cells per sample well. Cells with such phenotypes should be equated with Tc1 and Tc2 cells, respectively. Results are expressed as the mean (± S.D.) of three independent experiments with 5 mice per experiment (overall n = 15, * p < 0.05, ** p < 0.01, *** p < 0.001, control cells vs OCL-treated (10⁻⁶ or 10⁻⁷ M) cells (Student’s unpaired t-test)). Examples of dot plot cytograms show the distribution of IFN-γ- and IL-4-producing and non-producing cells within CD8⁺ T cell subset (E). Fluorescence minus one (FMO) controls were applied to confirm the gating strategy used to identify IFN-γ⁺ and IL-4⁺ cells.