Figure 3.

Effect of oclacitinib (OCL) on the number of IL-17-producing CD4⁺ and CD8⁺ T cells. The relative (A,C) and absolute count (B,D) of IL-17-producing CD4⁺ and CD8⁺ T cells were determined in cell cultures incubated with or without (control) OCL (10⁻⁶ M and 10⁻⁷ M). The relative count is expressed as a percentage of IL-17-producing cells within CD4⁺ and CD8⁺ T cells. The absolute count represents the number of IL-17⁺CD4⁺ and IL-17⁺CD8⁺ T cells per sample well. Cells with such phenotypes should be equated with Th17 and Tc17 cells, respectively. Results are expressed as the mean (±S.D.) of three independent experiments with 5 wells per experiment (overall n = 15). Examples of dot plot cytograms show the distribution of IL-17-producing and non-producing cells within CD4⁺ and CD8⁺ T cell subset (E). Fluorescence minus one (FMO) controls were applied to confirm the gating strategy used to identify IL-17⁺ cells.