Figure 4.

Effect of oclacitinib (OCL) on the number of IL-10-producing CD4⁺ and CD8⁺ T cells. The relative (A,C) and absolute count (B,D) of IL-10-producing CD4⁺ and CD8⁺ T cells were determined in cell cultures incubated with or without (control) OCL (10⁻⁶ M and 10⁻⁷ M). The relative count is expressed as a percentage of IL-10-producing cells within CD4⁺ and CD8⁺ T cells. The absolute count represents the number of IL-10⁺CD4⁺ and IL-10⁺CD8⁺ T cells per sample well. Results are expressed as the mean (±S.D.) of three independent experiments with 5 mice per experiment (overall n = 15, * p < 0.05, ** p < 0.01, *** p < 0.001, control cells vs OCL-treated (10⁻⁶ or 10⁻⁷ M) cells (Student’s unpaired t-test), or OCL 10⁻⁶-treated vs OCL-10⁻⁷-treated cells (one way ANOVA with Holm-Sidak multiple comparison test)). Examples of dot plot cytograms show the distribution of IL-10-producing and non-producing cells within CD4⁺ and CD8⁺ T cell subset (E). Fluorescence minus one (FMO) controls were applied to confirm the gating strategy used to identify IL-10⁺ cells.