Figure 7.

Gating strategy for flow cytometric data analysis and calculation of the absolute cell counts of lymphocyte subsets. Lymphocytes were identified based on forward and side scatter (FSC/SSC) properties, and then gated for expression of CD4 or CD8 surface receptors. Subsequently, IL-4-, IL-10-, IL-17- and IFN-γ-producing cells as well as CD49b⁺CD223⁺-expressing and 5-bromo-2’-deoxyuridine(BrdU)-incorporating cells were identified within particular T cell subsets. Absolute cell counts of lymphocyte subsets (i.e., number of cells from particular subpopulations per sample well) were calculated using the dual platform method, as shown above.