Table 1.

The staining combinations, characteristics of monoclonal antibodies and evaluated parameters.

Marker	Fluorochrome	Clone	Isotype	Evaluated Parameters
CD4	FITC	H129.19	IgG2a, κ	The percentage and absolute count of: ➢IL-4−IFN-γ+CD4+ T cells (Th1 cells) ➢IL-4+IFN-γ−CD4+ T cells (Th2 cells) ➢IL-4−IFN-γ+CD8+ T cells (Tc1 cells) ➢IL-4+IFN-γ−CD8+ T cells (Tc2 cells) ➢IL-10+CD4+ T cells ➢IL-10+CD8+ T cells
CD8a	APC-Cy7	53-6.7	IgG2a, κ
IL-4	PE-CF594	11B11	IgG1
IL-10	APC	JES5-16E3	IgG2b
IFN-γ	AF700	XMG1.2	IgG1,κ
CD4	FITC	H129.19	IgG2a, κ	The percentage and absolute count of: ➢IL-17+CD4+ T cells (Th17 cells) ➢IL-17+CD8+ T cells (Tc17 cells)
CD8a	APC-Cy7	53-6.7	IgG2a, κ
IL-17	PerCP-Cy5.5	TC11-18H10	IgG1, λ
CD4	FITC	H129.19	IgG2a, κ	The percentage and absolute count of: ➢CD49b+CD223+CD4+ T cells (Tr1 cells)
CD49b	PE-CF594	DX5	IgM, κ
CD223	BB700	C9B7W	IgG1, κ
CD4	FITC	H129.19	IgG2a, κ	The percentage of: ➢BrdU-incorporating CD4+ and CD8+ T cells (i.e., proliferating cells)
CD8a	APC-Cy7	53-6.7	IgG2a, κ
BrdU	APC	BU1/75	IgG2a