Table 2.
Predicted and known cleavages rates, sites and effector protease identities for multiplexed peptides pepA–pepE used in this study. Predictions were derived from [21]. Cleavage rates adapted taken from [22,23].
| Peptide ID | Sequence | Sequence Used for Query | Aspartic | Cysteine | Metallo | Serine | Known Cleavage & Rates (M-1 S-2) | Predicted Cleavage Identity |
|---|---|---|---|---|---|---|---|---|
| PepA (PEPDAB005) |
Dabcyl-Leu-Ala-Gln-Ala-(Homo phenylalanine)-Arg-Ser-Lys(5-FAM)-NH2 | LAQAFRSK | X QAFR|SK |
X LAQA|FRSK |
X AQAF|RSK |
MMP2 (3.2 × 105) MMP8 (1.4 × 105) MMP9 (2.2 × 105) |
Cys: cathepsin K (C01.036) Met: matrix metallopeptidase-9 (M010.004) Ser: chymotrypsin A (cattle-ty)pe) |
|
| PepB (PEPDAB008) |
Dabcyl-Pro-Cha-Gly-Cys(Me)His-Ala-Lys(5-FAM)-NH2 | PLGCHAL | X PLG|CHAL |
MMP2 (2.9 × 104) MMP8(2.4 × 104) MMP9 (8.5 × 105) |
Met: matrix metallopeptidase-2 (M10.003) | |||
| PepC (PEPDAB010) |
Dabcyl-Ser-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Lys(5-FAM)-NH2 | SPLAQAVRSSK | X QAVR|SSK |
X LAQA|VRS |
X AQAV|RSSK &SPL|AQAV |
MMP2 (1.7 × 105) MMP8 (2.6 × 104) MMP9 (6.0 × 105) |
Cys: cathepsin K (C01.036) Met: matrix metallopeptidase-9 (M10.004) Ser: elastase-2 (S01.131)/cathepsin G(S01.133) Other: proyl peptidase |
|
| PepD (PEPDAB013) |
Dabcyl-His-Gly-Asp-Gln-Met-Ala-Gln-Lys-Ser-Lys(5-FAM)-NH2 | HGDQMAQKSK | X HGDQ|MAQK |
X HGDQ|MAQK |
MMP2 (2.4 × 103) MMP8 no activity MMP9 no activity |
Cys: cathepsin K (C01.036) Predicted MMP20 activity |
||
| PepE (PEPDAB014) |
Dabcyl-Glu-His-Ala-Asp-Leu-Leu-Ala-Val-Val-Ala-Lys(5-FAM)-NH2 | EHADLLAVVAK | X DLLA|VVAK |
X EHAD|LLAV |
X ADLL|AVVA |
MMP2 (6.3 × 103) MMP8 (4.8 × 103) MMP9 no activity |
Asp: cathepsin D/cathepsin E Met: matrix-metallopeptidase-2 (M10.003), Ser: cathepsin G (S01.133) |