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. 2021 Sep 10;8(9):190. doi: 10.3390/vetsci8090190

Figure 3.

Figure 3

Timeline for hormone treatments administered to synchronize and resynchronize embryo recipient mares for embryo transfer. Cyclic: cyclic mares having ovulation confirmed after induction of ovulation (follicle ≥35 mm) with histrelin acetate (250 µg); LAP4: acyclic mares treated with estradiol-17β for 3 days (E2-3d) and then administered a single dose of long-action progesterone (LAP4, 1.5 g); IPRD: acyclic mares treated with E2-3d and then given an IPRD containing 1 g of natural progesterone; RE-IPRD: acyclic mares synchronized as in the IPRD group but not used for embryo transfer, then 8 to 15 days later (i.e., D12–D23 after the first synchronization), the mares were resynchronized with E2-3d and IPRD for 4–8 days. Embryo transfers (ET) were performed between 4 and 8 days after ovulation or progesterone treatment. Pregnancy diagnosis (PD) was performed five days after ET (Days 9–13). Pregnant embryo recipient mares from groups LAP4, IPRD, and RE-IPRD received weekly injections of LAP4 at PD. Pregnant mares in Groups IPRD and RE-IPRD had the device removed three days after the first PD.