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. 2021 Aug 27;13(9):599. doi: 10.3390/toxins13090599

Figure 2.

Figure 2

Characterization of ArtABHIS purified from E. coli. (A) binding (at 4 °C) and uptake (at 37 °C) of 50 μg/mL ArtABHIS after 1 h on Vero cells using anti-6XHIS (α-HIS) and FITC-conjugated secondary antibodies (negative = no toxin control, scale bar = 10 μm), (B) representative ELISA dilution series of ArtABHIS on ganglioside GM1 and fetuin (negative = no toxin control), and (C) average titers of fetuin and GM1 ELISAs of ArtABHIS using α-HIS and CT using anti-CT (α-CT) antibody. Results show the mean +/− SE (n = 4). A two-tailed, unpaired t-test was performed for comparison within each toxin group (ArtABHIS fetuin to GM1 * p = 0.0487; CT fetuin to GM1 * p = 0.0262).