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Purification of ArtAB and ArtB from E. coli. (A) plasmid pBM006 for expression of ArtAB, (B) SDS-PAGE of ArtAB purified with fetuin, showing increasing salt fractions (1: flow through, 2: 0.1 M NaCl, 3: 0.1 M MgCl2, 4: 0.5M NaCl, 5: 0.5M MgCl2, 6: 1 M NaCl). (C) SDS-PAGE of the ArtAB elution purified with d-galactose, and boiled/unboiled samples, (D) plasmid pLC001 for expression of ArtB and (E) SDS-PAGE of the ArtB elution purified on d-galactose, boiled and unboiled samples.
