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. 2021 Aug 27;13(9):599. doi: 10.3390/toxins13090599

Figure 4.

Figure 4

Metabolic and cytotoxic activity of ArtAB and CT on epithelial cells in vitro. (A) Resazurin metabolic assays and (B) crystal violet cytotoxicity assays on CHO cells, and (C) resazurin and (D) crystal violet assays on Vero cells. Purified ArtAB was incubated with cells at 2, 5, or 10 μg per 200 μL well for 4, 24, 48, or 72 h at 37 °C. Results are reported as the percent of vehicle control activity, and toxin groups at each time point are compared to media alone (also reported as the percent of vehicle control activity) using a one-way analysis of variance (ANOVA) and Tukey’s HSD. Stars above the bar indicate a significant difference from media alone at that time point (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).