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Direct use of tropoelastin (TE) impeded tube formation. (A) Quantitative summary of mesh numbers (Mesh #) in the absence (gray) or presence (black) of 0.2 µg/mL tropoelastin at 4, 5, and 6 h post-seeding. Tube formation assay was performed in a 96-well plate and images were taken at 6 h post-seeding. Representative tube formation in (B) EBM (referring to EBM-2) and (C) EBM + tropoelastin. Asterisks show * p < 0.05, ** p < 0.01. Bars represent SD.
