Figure 3.

6S/nLNP treatment alters fecal metabolites. (a) Representative UPLC-MS (ESI+, Q-TOF) total ion current (TIC) profiles of fecal metabolites before (day 0) and after (day 5, day 15) 6S/nLNP treatment. (b) Cloud plot demonstrating upregulated MS features (green; 1590 of 1844) and downregulated MS features (red; 254 of 1844) after five days of 6S/nLNP treatment (day 15 sample). (c) Venn diagram of disappeared or appeared fecal metabolites before and after 6S/nLNP treatment, as detected by LC-MS. Twenty-four metabolites disappeared and 60 new metabolites appeared after 5 days of treatment (day 15 sample). (d) Heatmap summary of the numerous significantly upregulated metabolites on day 15 induced by a five-day 6S/nLNP treatment and their possible molecular weights (MW.).