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. 2021 Sep 18;22(18):10107. doi: 10.3390/ijms221810107

Figure 4.

Figure 4

Downregulation of DUB3 is associated with PTC596-induced Mcl-1 downregulation and apoptosis. (A) Caki cells were transfected with HA-ubiqutin (HA-Ub) plasmid and treated with MG132 (0.5 μM) and PTC596 (10 nM). Mcl-1 ubiquitination was detected by Western blotting using HRP-conjugated anti-Ub antibody; (B,C) Caki cells were treated with PTC596 (5, 10 nM) for 24 h; (D–E) Caki cells were transfected with Cont, BMI-1 (D) or DUB3 siRNA (E) for 24 h; (F) Caki cells were transfected with Caki/Vec or Caki/DUB3 plasmids and then treated with PTC596 (10 nM) for 24 h. The sub-G1 population and protein expression were measured by flow cytometry (F) and Western blotting (A–F). The band intensity was examined using Image J (D,E). * p < 0.01 compared to the control siRNA. # p < 0.05 compared to the PTC596 in Caki/Vec cells.