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. 2021 Sep 10;22(18):9818. doi: 10.3390/ijms22189818

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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TPM4 and p63 expression in differentiating myocytes. (A,B) Identification of p63 (red) positive nuclei in differentiating myocytes. Only some mono-nucleated cells (stars) are positive. (C) Enlargement of mononucleated cells shows fibrillar distribution of TPM4 (green) and perinuclear localisation of p63 (red), probably identifying the time of the transport from ER to nucleus (arrow and stars). DAPI (blue) was used for nuclei staining. Laser wavelengths as the previous figure. Bars = 10 µm.