FIG. 4.

Northern blot analysis of D-HIT, IFN-α, or interferon-inducible (6-16) gene transcripts in B cells. Poly(A)⁺ RNA (5 μg) from uninduced or TPA- and SB-induced cells (as indicated) was loaded onto gels and detected by hybridization with ³²P-radiolabelled probes from EBV IR4 DNA (A) or cDNAs for IFN-α (B) or 6-16 (B and C). The sources of the RNA and the nature of the inducing agents (TPA and SB), if used, are indicated, and predicted locations of the individual RNAs are marked. Although the levels of IFN-α in panel B are low, they mimic those observed for D-HIT (A) and 6-16 (B), where increased levels are observed with TPA-treated cells and even higher levels are detected with SB-treated cells.