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. 2021 Sep 8;22(18):9696. doi: 10.3390/ijms22189696

Figure 2.

Figure 2

Cyclosporine A (CsA) and tacrolimus (Tac) impaired ECFC migration. (A) Representative images of DAPI-stained migrated ECFCs treated with CsA or Tac (0.01–10 µM) in a chemotaxis assay, scale bar 150 µm. (B) ECFCs showed a significantly lower directional migration in presence of CsA or Tac after 4 h. Numbers of DAPI-stained migrated cells on the bottom side of the membranes were counted in each picture, control group set as 1. n = 20. (C) CsA or Tac treatment (0.01–10 µM) of ECFCs diminished wound closure assessed as remigrated area after 18 h compared to control, control group set as 1. Representative images can be found in Supplemental Figure S2. n = 15–16. (D) CsA or Tac (1 µM or 10 µM) did not lead to significantly higher LDH release after 18 h, positive control set as 100%, n = 9. Con—control; CsA—cyclosporine A; Tac—tacrolimus. ** p < 0.01, *** p < 0.001.