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. 2021 Aug 24;10(9):1759. doi: 10.3390/plants10091759

Table 4.

Overview of categorical plant-level phenotypes and description of the according scoring metrics.

Plant-Level Phenotype Scoring Metric Description
Growth stage BBCH scale Phenological growth stage; very important to record at mature phenotyping
Seed shattering 1,3,5,7 Grain persistence in the plant at physiological maturity. Assessing how easy seeds fall off the panicle upon light touch: 1 = no seeds falling to 7 = majority of seeds falling
Panicle shape 1,3,5 Classified into one of the three categories: glomerulate, intermediate, or amarantiform
Panicle density 1,3,5,7 Scored from 1 = lax (loose) with panicle axes easily visible to 7 = tight and compact panicles
Panicle leafiness 1,3,5,7 Scored from 1 = no leaves to 7 = many leaves
Panicle color 13,4,15,16,5,7 Categorized according to the color phenotyping card
Stem color 13,4,15 Categorized into green (13), red (4), or no pigmentation (15)
Stem striae 0,1 Presence (1) absence (0) scoring of stem streaks or stripes
Axil pigmentation 0,1 Presence (1) absence (0) scoring of pigmented axils
Stem leaf shape 1,2 Leaves of the stem are categorized into two groups: rhomboidal (1) and triangular (2)