Table 6.

Washington State University Sustainable Seed Systems lab NIR calibration (V3) metrics. The range, minimum (min), and maximum (max) are calculated using reference data for quinoa samples included in the calibration (n = 175). Calibration prediction accuracy metrics are reported as an average measure of 8-fold cross validation in triplicate.

	Stats from WSU Calibration V3 Data (g 100g−1 Protein)
	Range	Min	Max	RMSECV	SECV	Robust SECV	RPDCV	R2CV
Alanine	1.99	2.89	4.88	0.022	0.022	0.018	3.036	0.892
Arginine	4.68	4.58	9.25	0.053	0.053	0.044	4.308	0.946
Aspartic acid	3.22	5.51	8.73	0.039	0.040	0.036	3.768	0.930
Cysteine	0.76	1.31	2.07	0.010	0.010	0.010	3.188	0.902
Glutamic acid	7.04	8.22	15.26	0.093	0.093	0.086	3.802	0.931
Glycine	1.33	4.78	6.11	0.041	0.041	0.036	2.447	0.834
Histidine	1.05	1.96	3.01	0.015	0.015	0.014	4.564	0.952
Isoleucine	1.51	2.89	4.41	0.021	0.022	0.019	3.392	0.913
Leucine	2.55	4.3	6.85	0.031	0.032	0.029	3.473	0.917
Lysine	3.14	3.45	6.59	0.029	0.029	0.033	3.290	0.908
Methionine	1.15	1.31	2.46	0.012	0.012	0.009	2.955	0.886
Phenylalanine	1.57	2.71	4.28	0.019	0.019	0.018	3.889	0.934
Proline	1.68	2.80	4.48	0.023	0.023	0.018	2.556	0.847
Serine	1.39	2.89	4.28	0.019	0.019	0.016	3.176	0.901
Taurine	1.96	0.82	2.79	0.012	0.012	0.009	1.669	0.645
Threonine	1.60	2.43	4.02	0.017	0.017	0.016	3.015	0.890
Tryptophan	0.93	0.55	1.48	0.012	0.012	0.009	1.681	0.647
Tyrosine	0.93	2.12	3.05	0.014	0.014	0.013	3.393	0.913
Valine	1.84	3.36	5.20	0.024	0.024	0.023	3.260	0.906
Hydroxylysine	0.18	0.05	0.23	0.004	0.004	0.003	1.591	0.605
Hydroxyproline	0.93	0.29	1.21	0.010	0.010	0.011	1.821	0.699
Stats from WSU calibration V3 data (g 100g−1 sample)
Crude protein	11.95	6.82	18.77	0.394	0.395	0.406	5.521	0.967
Ash	3.32	2.21	5.53	0.154	0.154	0.129	3.084	0.895
Crude fat	6.95	0.00	6.95	0.310	0.311	0.316	3.883	0.934
Crude fiber	13.67	1.44	15.11	0.442	0.443	0.377	4.904	0.958
Moisture	3.76	6.41	10.17	0.183	0.183	0.159	6.579	0.977
TotalAA	10.06	5.84	15.90	0.413	0.413	0.328	4.018	0.938
	Range	Min	Max	RMSECV	SECV	Robust SECV	RPDCV	R2CV

Hydroxylysine and hydroxyproline are poorly predicted. Lanthionine and ornithine were eliminated from the calibration owing to limited lab analysis. RMSECV = root mean square error of cross validation; SECV = standard error of cross validation; RPDCV = ratio of reference data standard deviation to standard error of prediction; R2CV = coefficient of determination of cross validation.