Figure 1.

Acute busulfan exposure alters testes in male mice. (A) Body, seminal vesicle, epididymis, and testis weights of C57BL/6J males 4 or 8 weeks after busulfan or vehicle treatments. (B) Representative micrographs of hematoxylin/eosin-stained testes of C57BL/6J males 4 or 8 weeks after busulfan or vehicle treatments. (C) The number of normal seminiferous tubules of C57BL/6J males treated with the vehicle or busulfan (4 or 8 weeks after treatment). For all panels, the data were obtained from at least three independent experiments; the number of animals per group is indicated in brackets below the bars of the graphs. The data are expressed as the means ± SEM. Statistical analysis: ** p < 0.01, and *** p < 0.001 vs. the vehicle group. Veh.: vehicle and Bu: busulfan.