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. 2021 Sep 13;10(9):2403. doi: 10.3390/cells10092403

Figure 4.

Figure 4

Busulfan treatment leads to the alteration of germ cell numbers through apoptosis in the GC1spg cell line. (A) Number of adherent cells after 24 or 48 h of treatment with vehicle or Bu in GC1spg cells. (B) Number of adherent cells after 24 or 48 h of treatment with vehicle or Bu in GC2spd (ts) cells. (C) The relative number of TUNEL-positive of vehicle- or Bu-treated GC1spg cells after 24 h. For all panels, the data were obtained from at least three independent experiments. The data are expressed as the means ± SEM. Statistical analysis: * p < 0.05, ** p < 0.01 vs. the vehicle group. Veh.: vehicle and Bu: busulfan.