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. 2021 Sep 9;10(9):2363. doi: 10.3390/cells10092363

Figure 3.

Figure 3

Immunoblotting of a recombinant VDE (positive control, with a MW slightly affected because of the His-tag) and VDE in intact TMs and in granum and stroma membrane particles. Proteins were separated and VDE detected using SDS-PAGE/western blotting. Total Chl contents loaded in the wells were 2 and 20 µg for intact TMs subchloroplast particles, respectively. MW of the bands, 49.5 kDa (upper) and 44.0 kDa (lower). Images of two equally treated blots were processed for granum and stroma TMs. Exposure times: 38 s (intact and granum TMs) and 30 s (stroma TMs).