Table 8.
Bacillus velezensis strain B25 effects on mortality, biomass increase, and fecundity of Eisenia fetida. NOEC stands for no observed effect concentration and EC50 for half maximal effective concentration.
| Product Tested | Bacillus velezensis Strain B25 (1 × 108 cfu·g−1) | |||
|---|---|---|---|---|
| Assay Element/Substrate | Eisenia fetida/Artificial Soil | |||
| Application Rates (kg·ha−1) | Average Mortality at 28 Days (%) * | Biomass Increase (Average %) ** | Fecundity | |
| Average Number per Adult | Effects (%) *** | |||
| Control | 0 | +80.69 | 17.84 | NA |
| 1 | 1.30 (NS) | +87.20 (NS) | 16.93 (NS) | −5.63 (NS) |
| 2 | −3.90 (NS) | +86.84 (NS) | 14.33 (NS) | −20.14 (NS) |
| 10 | −3.90 (NS) | +85.51 (NS) | 17.15 (NS) | −4.39 (NS) |
| NOEC (kg·ha−1) | 10 | |||
| EC50 (kg·ha−1) | >10 | |||
* Based on the average of dead or not retrieved organisms, positive values indicate a higher mortality compared to control group, negative values indicate a lower mortality compared to control group. ** Positive values indicate an increase of biomass in comparison with day 0. *** Positive values indicate a higher fecundity compared to control group; negative values indicate a lower fecundity compared to control group. Significant differences (S) or non-significant differences (NS) based on Kruskal–Wallis test for mortality values and ANOVA for biomass increase and fecundity. NA = Not applicable.