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. 2021 Sep 7;10(9):1426. doi: 10.3390/antiox10091426

Figure 4.

Figure 4

Effects of treatment with antibiotics on SLE-linked endothelial dysfunction, NADPH oxidase activity and immune cell aorta infiltration in imiquimod (IMQ) mice. (A) Acetylcholine (Ach)-induced relaxation responses in aortas pre-contracted by U46619 (10 nM), with or without the Rho kinase inhibitor Y27632 (1 µM); control (CTR), IMQ and IMQ-groups treated with Vancomycin (VANCO) or our cocktail of antibiotics (MIX). (B) Aortic responses induced by Ach in the absence or in the presence of the specific pan-NOX inhibitor VAS2870 (1 µM) and aortic NADPH oxidase activity determined using a lucigenin-enhanced chemiluminescence. (C) Aortic responses induced by Ach in the absence or in the presence of anti-IL-17a antibody (10 µg·L) and aortic infiltration of Th17 assessed with flow cytometry. Data is represented as means ± SEM. The concentration-response curves to Ach were analysed by two-way ANOVA with the Tukey’s multiple comparison test. The rest of the variables were tested with one-way ANOVA and Tukey post hoc test. † p < 0.01, ‡ p < 0.001 in comparison with CTR; * p < 0.05, † p < 0.01, ‡ p < 0.001 in comparison with IMQ.