Figure 6.

Activity of neutrophils isolated 4 and 10 months after implantation (experimental: 8 sheep) compared to control groups of neutrophils isolated at the same time points from animals from control groups (control: 4 sheep). The neutrophil response to Ti implant insertion, with or without stimulation with AMP extract; (a) MPO release from neutrophils, (b) NO generation, (c) superoxide generation assessed at two time points: 4 and 10 months after implantation. Two measurements, after 30 min and 20 h incubation at 37 °C and 5% CO₂ were conducted. Data present mean values ± SE of at least three replicates for each bar. * p < 0.05 compared to cultures of neutrophils from the control group at the same time points. Legends: C0—control group before surgery, E0—experimental group before surgery, C 4m—control group 4 months after, E 4m—experimental group 4 months after, C 10m—control group 10 months after, E 10m—experimental group 10 months after insertion of a titanium implant.