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. 2021 Sep 13;22(18):9863. doi: 10.3390/ijms22189863

Figure 2.

Figure 2

Decrease of LasR-LBD solubility by artificial sweeteners monitored by Western blotting of the soluble fraction from the LasR-LBD overexpression in E. coli BL21 strain in the presence of (A) aspartame, (B) saccharin, and (C) sucralose. All concentrations presented are final concentrations. The final concentration of 3-oxo-C12-HSL used was 100 nM. Integrated density is the sum of the values of the pixels within the protein band determined using ImageJ software [22]. **** p < 0.0001. Values represent mean ± SD, n = 3. Structures were drawn using MedChem Designer, Version 5.5 (Simulations Plus Inc., Lancaster, CA, USA).