Table 3.
Relative abundance of bacterial families in the ileal chymus of broiler chickens as affected by dietary treatments and age (%).
| Family | Ileal Chymus | FDR p-Values | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Dietary Treatment | Age of Birds | Mean (Dietary Treatment) | |||||||
| d 7 | d 14 | d 21 | d 40 | Dietary Treatment | Age | Int. | |||
| Lactobacillaceae | C | 79.43 A | 59.01 | 63.68 | 60.68 | 65.70 | 0.710 | 0.030 | |
| Br | 49.84 B | 45.53 | 69.02 | 76.02 | 60.10 | ||||
| Sy | 75.20 AB | 56.34 | 51.58 | 67.25 | 62.59 | ||||
| Mean (Age) | 68.16 | 53.62 | 61.43 | 67.99 | 0.130 | ||||
| Enterococcaceae | C | 1.74 B | 0.16 | 1.42 | 1.14 | 1.12 | 0.510 | 0.011 | |
| Br | 18.17 A | 0.32 | 1.88 | 0.92 | 5.32 | ||||
| Sy | 13.76 AB | 1.69 | 2.19 | 1.76 | 4.85 | ||||
| Mean (Age) | 11.22 a | 0.72 b | 1.83 b | 1.27 b | 0.001 | ||||
| Lachnospiraceae | C | 6.88 | 6.68 | 1.02 | 0.15 | 3.68 | 0.640 | 0.530 | |
| Br | 7.55 | 6.28 | 0.70 | 0.19 | 3.68 | ||||
| Sy | 0.49 | 1.94 | 2.99 | 0.35 | 1.44 | ||||
| Mean (Age) | 4.97 | 4.96 | 1.57 | 0.23 | 0.100 | ||||
| Enterobacteriaceae | C | 0.80 | 1.76 | 0.63 A | 0.18 | 0.85 | 0.320 | 0.079 | |
| Br | 9.45 | 16.68 | 0.08 B | 0.17 | 6.60 | ||||
| Sy | 0.07 | 1.62 | 0.14 AB | 0.41 | 0.56 | ||||
| Mean (Age) | 3.44 | 6.69 | 0.28 | 0.25 | 0.065 | ||||
| Ruminococcaceae | C | 4.81 | 9.04 | 1.17 | 0.07 | 3.77 | 0.830 | 0.950 | |
| Br | 3.51 | 4.77 | 0.42 | 0.10 | 2.20 | ||||
| Sy | 1.31 | 5.85 | 2.23 | 0.14 | 2.38 | ||||
| Mean (Age) | 3.21 ab | 6.55 a | 1.27 b | 0.10 b | 0.025 | ||||
| Mitochondria | C | 0.12 | 0.08 | 0.02 | 0.00 | 0.05 | 0.600 | 0.450 | |
| Br | 0.28 | 0.27 | 0.02 | 0.01 | 0.14 | ||||
| Sy | 0.51 | 0.25 | 0.01 | 0.00 | 0.19 | ||||
| Mean (Age) | 0.30 a | 0.20 ab | 0.01 b | 0.00 b | 0.002 | ||||
| Streptococcaceae | C | 1.71 | 4.66 | 8.93 | 12.88 | 7.04 | 0.600 | 0.820 | |
| Br | 1.26 | 4.46 | 5.93 | 6.84 | 4.62 | ||||
| Sy | 0.16 | 4.71 | 6.50 | 7.79 | 4.79 | ||||
| Mean (Age) | 1.04 b | 4.61 b | 7.12 a | 9.17 a | 0.001 | ||||
| Clostridiaceae_1 | C | 0.65 | 0.19 | 6.20 | 0.44 | 1.87 | 0.600 | 0.420 | |
| Br | 0.75 | 1.35 | 5.83 | 0.11 | 2.01 | ||||
| Sy | 0.65 | 11.47 | 6.45 | 1.26 | 4.96 | ||||
| Mean (Age) | 0.68 | 4.34 | 6.16 | 0.61 | 0.075 | ||||
| Peptostreptococcaceae | C | 1.08 | 10.57 | 0.31 | 8.78 | 5.18 | 0.860 | 0.950 | |
| Br | 1.08 | 11.97 | 0.96 | 6.64 | 5.16 | ||||
| Sy | 0.04 | 7.22 | 2.17 | 6.87 | 4.07 | ||||
| Mean (Age) | 0.73 b | 9.92 a | 1.15 b | 7.43 a | 0.001 | ||||
| Corynebacteriaceae | C | 0.05 | 0.12 | 5.63 | 3.22 | 2.25 | 0.600 | 0.770 | |
| Br | 0.56 | 0.07 | 3.49 | 2.01 | 1.53 | ||||
| Sy | 0.09 | 0.25 | 8.08 | 4.49 | 3.23 | ||||
| Mean (Age) | 0.23 b | 0.15 b | 5.73 a | 3.24 ab | 0.001 | ||||
| Leuconostocaceae | C | 0.05 | 0.30 | 2.63 | 0.75 | 0.93 | 0.600 | 0.230 | |
| Br | 0.40 | 0.28 | 1.69 | 0.48 | 0.71 | ||||
| Sy | 0.02 | 0.21 | 4.53 | 0.72 | 1.37 | ||||
| Mean (Age) | 0.16 b | 0.26 b | 2.95 a | 0.65 b | 0.001 | ||||
| Staphylococcaceae | C | 0.04 | 0.81 | 5.72 | 2.41 | 2.24 | 0.860 | 0.880 | |
| Br | 0.24 | 0.19 | 8.02 | 0.99 | 2.36 | ||||
| Sy | 0.08 | 0.56 | 7.85 | 2.57 | 2.76 | ||||
| Mean (Age) | 0.12 b | 0.52 b | 7.20 a | 1.99 b | 0.001 | ||||
| Erysipelotrichaceae | C | 0.04 | 0.21 | 0.19 | 6.38 | 1.71 | 0.770 | 0.360 | |
| Br | 0.21 | 0.13 | 0.14 | 3.38 | 0.97 | ||||
| Sy | 0.04 | 0.98 | 0.68 | 2.04 | 0.93 | ||||
| Mean (Age) | 0.10 b | 0.44 b | 0.34 b | 3.93 a | 0.001 | ||||
| Bacillaceae | C | 0.01 B | 0.03 | 0.02 | 0.002 B | 0.01 B | 0.007 | 0.370 | |
| Br | 0.02 B | 0.05 | 0.01 | 0.002 B | 0.02 B | ||||
| Sy | 0.14 A | 0.2 | 0.28 | 0.04 A | 0.16 A | ||||
| Mean (Age) | 0.06 | 0.09 | 0.10 | 0.02 | 0.230 | ||||
Bacterial family differences between groups were assessed using two-way ANOVA test, with Benjamini–Hochberg false discovery rate (FDR) correction. FDR-corrected p-values below 0.05 were considered significant. Dietary treatment effects at each sampling days were also compared with one-way ANOVA. The significance of Tukey’s HSD multiple group comparison’s post hoc tests was indicated at p ˂ 0.05. a, b: values within the mean (Age) rows with different lowercase letters were significantly different (p < 0.05). A, B: values within the mean (d 7, d 40) column with different capital letter superscripts were significantly different (p < 0.05). The table shows only those families for which the group average of relative abundance was higher than 1%. “Int.” means the FDR p-values of interaction between the two main factors, age, and dietary treatment.