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. 2021 Sep 3;13(9):1760. doi: 10.3390/v13091760

Table 3.

Amino acid changes in NS1 proteins from H5N1 IAV isolated in avian.

Position Amino Acid 1996–1997 1998–2000 2001–2005 2006–2010 2011–2015 2016–2020
55 R 75 65.38 1.5 0.84 0.32 3.04
E 25 34.62 96.98 94.1 87.93 96.34
Other 0 0 1.5 5.67 11.74 1.83
60 E 70 65.38 1.5 2.22 0.54 3
A 30 34.62 9.39 97.21 80.54 87.95
Other 0 0 0.1 0.55 18.91 9
63 H 60 3.84 1.07 0.18 0 2.41
Q 25 30.77 98.06 99.81 98.91 94.57
Other 15 65.38 0.86 0 1.08 3.01
87 P 75 65.38 3.11 0.83 2.17 4.89
S 25 34.61 93.23 98.98 97.5 96.34
Other 0 0 3.65 0.18 0.32 0
92 E 55 0 0.43 0.092 44.45 37.35
D 45 100 99.46 99.91 55.21 62.65
Other 0 0 0.1 0 0.32 0
103 L 60 3.8 1.18 0.092 0 2.4
F 25 26.92 97.42 97.4 99.78 94.57
Other 15 69.23 1.39 2.5 0.21 3
106 I 60 11.53 1.29 0.28 0 0
M 40 84.61 98.71 99.72 100 100
Other 0 3.8 0 0 0 0
112 T 70 65.38 7.95 48.84 6.1 5.42
A 30 34.61 91.73 44 13.49 54.82
Other 0 0 0.32 7.24 80.41 39.76
118 R 85 15.38 6.87 1.95 4.13 40.96
K 15 84.61 93.12 98.05 95.86 59.03
127 D 55 0 0.43 0 0 0
N 30 38.46 18.8 2.78 3.99 53
Other 15 61.54 80.77 97.21 95.32 46.99
139 N 65 7.69 1.93 1.2 2.61 69.88
D 35 92.3 96.88 96.75 90.53 29.51
Other 0 0 1.18 2 6.85 0.6
143 A 65 3.84 0.86 0.37 0.11 0
T 35 96.15 96.45 91.37 92.93 99.4
Other 0 0 2.68 8.25 7.07 0.6
152 D 55 7.69 1.07 0.092 0.32 1.2
E 45 92.31 98.17 98.98 99.02 98.8
Other 0 0 0.75 0.93 0.64 0
171 E 55 0 0.75 0.27 0 0
D 20 38.46 28.76 83.22 8.27 62.65
Other 25 61.54 70.49 93.21 91.73 37.35
202 T 55 0 0.86 1.76 1.63 0
A 45 100 97.85 97.86 98.14 100
Other 0 0 0.96 0.37 0.22 0
212 S 55 0 1.61 0.092 0 0.6
P 45 100 22.31 5 12.33 43.97
Other 0 0 76.07 94.9 87.66 55.42
223 E 61.1 0 0.54 0.75 0.11 0
A 38.89 100 99.46 99.25 99.89 100
228 P 61.1 0 2 3.11 1.21 6
S 38.89 100 97.73 93.69 98.79 94
Other 0 0 0.21 0.092 0 0

Frequencies of identified mutations in H5N1 IAV NS1 proteins isolated from avian at the indicated time periods. Publicly available sequences in the Influenza Research Database (https://www.fludb.org/brc/home.spg?decorator=influenza (accessed on 23 June 2020)) were downloaded and the frequencies represented according to the year of isolation. The original amino acid present in 1996–1997 H5N1 IAV isolates is depicted first, whereas the amino acid most frequently found nowadays is depicted second. The numbers of sequences available at the Influenza Research Database are 20, 36, 930, 1074, 920, and 164, for periods 1996–1997, 1998–2000, 2001–2005, 2006–2010, 2011–2015, and 2016–2020, respectively.