Figure 2.

The effect of melatonin on the expressions of cytokines in MDBK cells. MDBK cells were inoculated with BVDV (MOI = 5) in the presence or absence of MT. Total RNA was extracted from cell lysates at 36 h post-infection. The relative expression of (A) IL-6 mRNA; (B) IL-1β mRNA; (C) IFN-α mRNA; (D) IFN-β mRNA; (E) Mx 1 mRNA was assessed by RT-qPCR. The values in each column represent the mean ± SEM of three individual experiments detected in triplicate. * p < 0.05; ** p < 0.01; *** p < 0.001.