Figure 4.
Effects of BV on proinflammatory cytokines and chemokines in gouty rat. (A) Reverse transcriptase PCR of proinflammatory cytokines (TNF-α, IL-1β, IL6, COX2 and iNOS) and chemokines (MIP-1α, MIP-1β, MCP-1, GRO-α, MIP-2α) (at 24 h) in normal, vehicle-treated, BV-treated, and Col-treated rats (n = 3/group). (B,C) Quantitative analysis of reverse transcriptase PCR. (D) Western blots of iNOS and COX2 at 24 h in normal, vehicle-treated, BV-treated, and Col-treated rats (n = 3/group). (E) Quantitative analyses of western blots. (F) Immunohistochemistry for iNOS and COX2 in synovium tissue of ankle. Scale bar, 50 μm. Data are presented as means ± SD. * p < 0.05 versus vehicle (one way ANOVA).