Table 2.

Nucleotide sequences of primers and conditions used for RT-PCR.

Target	Primer	Sequence	Annealing Temperature (℃)	Cycles
TNF-α	Forward	5′-CCC AGA CCC TCA CAC TCA GAT-3′	58	30
	Reverse	5′-TTG TCC CTT GAA GAG AAC CTG-3′
IL-1β	Forward	5′-GCA GCT ACC TAT GTC TTG CCC GTG-3′	52	35
	Reverse	5′-GTC GTT GCT TGT CTC TCC TTG TA-3′
COX-2	Forward	5′-CCA TGT CAA AAC CGT GGT GAA TG-3′	58	35
	Reverse	5′-ATG GGA GTT GGG CAG TCA TCA G-3′
iNOS	Forward	5′-CTC CAT GAC TCT CAG CAC AGA G-3′	59	30
	Reverse	5′-GCA CCG AAG ATA TCC TCA TGA T-3′
MIP-1α	Forward	5′-ACT GCC TGC TGC TTC TCC TAC A-3′	62	35
	Reverse	5′-AGG AAA ATG ACA CCT GGC TGG-3′
MIP-1β	Forward	5′-TCC CAC TTC CTG CTG TTT CTC T-3′	60	35
	Reverse	5′-GAA TAC CAC AGC TGG CTT GGA-3′
MCP-1	Forward	5′-TCA GCC AGA TGC AGT TAA CG-3′	55	30
	Reverse	5′-GAT CCT CTT GTA GCT CTC CAG C-3′
GRO-α	Forward	5′-CCG AAG TCA TAG CCA CAC TCA A-3′	65	35
	Reverse	5′-GCA GTC GTC TCT TTC TCC GTT AC-3′
MIP-2α	Forward	5′-AGA CAG AAG TCA TAG CCA CTC TCA AG-3′	32	35
	Reverse	5′-CCT CCT TTC CAG GTC AGT TAG C-3′
GAPDH	Forward	5′-AAC TTT GGC ATT GTG GAA GG-3′	50	25
	Reverse	5′-GGA GAC AAC CAG GTC CTC AG’