Figure 3.

The L2ATGko mutant-induced tumor growth at tails of the nude mice. More tail lesions (C) than muzzle lesions (A) were detected. Viral DNA was detected in the suprabasilar epidermis of muzzle lesions by in situ hybridization (ISH), and viral E4 protein was detected in the same regions of the muzzle lesions by immunohistochemistry (IHC) (B, 20×). DNA extracted from tail lesions induced by the L2 mutant maintained the ATG–ACG mutation. Input L2ATG mutant DNA was aligned with the amplified DNA from lesions (D).