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Viral transcripts (E4, L1, and L2) were detected in L1-L2ATGko infected tissues. Both wild-type (A) and L1-L2 mutant (B) infected tissues were examined for viral transcripts of E4, L1, and L2. All three transcripts (L1, L2, and E4) were found in both wild-type (Vaginal tissue) and L1-L2ATGko mutant (vaginal and tongue tissues). The mutation of L1-L2ATGko mutant was maintained in the lesions and confirmed by DNA sequencing following rolling circle amplification (C).
