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. 2021 Aug 24;13(9):1674. doi: 10.3390/v13091674

Figure 1.

Figure 1

THGP suppresses IFN-β induction in response to 3pRNA but not poly I:C, HT-DNA, and LPS. (A–D) ELISA of IFN-β levels at 24 h after stimulation with 3pRNA (A), polyI:C (B), HT-DNA (C), and LPS (D) in RAW264.7 cells pretreated with indicated concentrations of THGP for 24 h. Data are presented as mean and s.d. (n = 3). (E–H) Whole cell lysates at the indicated time after stimulation with 3pRNA (E), polyI:C (F), HT-DNA (G), and LPS (H) in RAW264.7 cells pretreated with THGP for 24 h, which were prepared and subjected to immunoblotting with anti-pTBK1, TBK1, pIRF-3, IRF-3, and β-actin antibodies. ** p < 0.01 vs. control. Data are representative of at least three independent experiments.