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. 2021 Sep 12;13(9):1812. doi: 10.3390/v13091812

Figure 2.

Figure 2

Isolation and identification of an FCV strain (SMU-B22-2020) in this study. (a) CRFK cells were inoculated with FCV SMU-B22-2020 and uninfected medium is shown at 12 h postinfection; (b) plaque assay for SMU-B22-2020 isolate with a dilution of 10−10 of virus stock using CRFK cells; (c) FCV particles with a diameter of approximately 30 nm were observed in cell supernatants by transmission electron microscopy (EM). Red arrows indicate the FCV particles; (d) the FCV isolate was further confirmed by RT-PCR with a 1300 bp target fragment. NC indicates the negative control.