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. 2021 Aug 27;9(9):959. doi: 10.3390/vaccines9090959

Figure 3.

Figure 3

Effect of RNA-TMEP transfection on human THP-1 cells. (A,B) THP-1 macrophages were transfected with RNA-TMEP or RNA-TMEPmod and at 3, 6 or 24 h post-transfection TMEP expression was analyzed by flow cytometry (A) or western blotting (B). In both cases, the detection was performed using a specific anti-FLAG antibody. Geometric mean fluorescence intensity (gMFI) values on the “live cells” gate were used to calculate the TMEP score by applying the formula: No. TMEP+ cells × gMFI/No. live cells. ** p < 0.01; *** p < 0.001. (C) Proinflammatory cytokine and chemokine profile induced at 6 h post-transfection by RT-qPCR in THP-1 cells transfected with RNA-TMEP, RNA-TMEPmod or RNA-luc (used as control). Results are expressed as the ratio of the gene of interest to the control previously normalized by HPRT mRNA levels. A.U.: arbitrary units. * p < 0.05; *** p < 0.001. Means and standard deviations of triplicate samples from one experiment are represented. Data shown are representative of two independent experiments performed.