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In vitro methylation activity and substrate specificity for his-AtHEN1 and his-MpHEN1. The his-AtHEN1 (A) and his-MpHEN1 (B). In vitro methyltransferase activity assay. Meth, the 21-nt position represents the methylated miRNA. UnMeth, the 20-nt position represents the unmethylated miRNA. (C) The substrate specificity evaluation of AtHEN1 and MpHEN1.
