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. 2021 Sep 15;13(9):1837. doi: 10.3390/v13091837

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The HC-Pro-mediated HEN1 activity inhibition. (A) The in vivo miR159 methylation status in wild-type TuMV (TuGR) and TuMV mutant (TuGK)-infected Col-0. (B) Evaluation of miRNA methylation status in P1/HC-Pro^R and P1/HC-Pro^K plants. The miRNA methylation status of 1-week-old plants was examined by oxidation/β-elimination, followed by small-RNA Northern blotting. (C) The in vivo miR166a methylation status in various mutant and transgenic plants. TAK1, the wild-type M. polymorpha, Col-0, wild-type Arabidopsis. hen1/heso1 mutant, the HEN1, and HESO1 double mutant. P1/HC-Pro^R, P1/HC-Pro^R plant.