Phylogenetic tree of HEN1 and HEN1 orthologs of different species of plant kingdom. Maximum likelihood (ML) tree of 22 HEN1 orthologs of green plants. MAGNESIUM-PROTOPORPHYRIN IX METHYLTRANSFERASE (CHLM) of Arabidopsis was used as the outgroup. Abbreviation of species names: Sm, Selaginella moellendorffii (Smoe109211); At, Arabidopsis thaliana (AT4G20910); Arabidopsis thaliana (AT4G20920); Os, Oryza sativa (BAJ16352); Atr, Amborella trichopoda (evm_27.model.AmTr_v1.0_scaffold00092.83); Pab, Picea abies (PAB00043215); Mp, Marchantia polymorpha (Mp3G16010); Pp, Physcomitrella patens (Pp3c1_60V3.4); Kf, Klebsormidium flaccidum (kfl00033_0220_v1.1); Cr, Chlamydomonas reinhardtii (Cre03.g191200); Vc, Volvox carteri (Vocar.0035s0143.1); Zm, Zea mays (GRMZM2G107457_T01); Sf, Sphagnum fallax (Sphfalx0066s0042.1, Sphfalx0001s0228.1); Sl, Solanum lycopersicum (Solyc02g070030.2.1); Pt, Populus trichocarpa (Potri.001G465500, Potri.011G163600); Gr, Gossypium raimondii (Gorai.010G144100); Tc, Theobroma cacao (Thecc1EG026937); Cpa, Carica papaya (evm.model.supercontig_166.41), Vv, Vitis vinifera (GSVIVG01021670001) and Arabidopsis thaliana, CHLM (AT4G25080). The bootstrap values are shown above the branches at the nodes. Arrowheads indicate duplicated events.