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. 2021 Aug 31;13(9):611. doi: 10.3390/toxins13090611

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The haemolysis activity of peptides was evaluated by using horse erythrocytes with peptide concentrations from 1 μM to 128 μM. Red blood cells treated with 0.1% Triton X-100 and PBS were used as the positive control and negative control, respectively. B1OS-D-L caused less than 10% haemolysis at its maximum MIC value (32 μM). Data were represented as the means ± standard deviation of three independent experiments. The statistical significance is indicated as * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001) versus negative control.