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. 2021 Sep 3;13(9):1765. doi: 10.3390/v13091765

Figure 2.

Figure 2

Regions of the FMDV IRES precipitate with the host protein DDX21. (a) Schematic diagram of FMDV IRES domains. (b) PK-15 cells in 100 mm cell culture dishes were transfected with 10 µg of Flag-DDX21. The lysate was collected in RIPA buffer and mixed with the in vitro synthesized biotinylated full-length FMDV IRES and domains D1–2, D3–5, D3, D4–5, D4, and D5. Following pulldown, beads were eluted with elution buffer, and 1× SDS loading buffer was added for Western blot analysis. (c) PK-15 cell in 100 mm cell culture dishes were transfected with 10 µg of Flag-nucleolin. The lysate was collected in RIPA buffer and mixed with the in vitro synthesized biotinylated full-length FMDV IRES and domains D1–2, D3–5, D3, D4–5, D4, and D5. Following pulldown, beads were eluted with elution buffer, and 1× SDS loading buffer was added for Western blot analysis.