Table A1.
Novel mycoviruses discovered by metatranscriptomics approach from sunflower (RdRp: RNA-dependent RNA polymerase). The SF1 library was constructed from RNA extracts of phomopsis-infected leaves, while the SF2 library was constructed from rust-infected leaves.
| Contig Name | SF 1 Read Counts |
SF 2 Read Counts |
Abbreviation | Genbank Accession | Genome | Contig Length (nt) | Identity (%) | Putative Function (Most Similar Virus) |
|---|---|---|---|---|---|---|---|---|
| Helianthus annuus alphaendornavirus | 16,590 | 36 | HaEV1 | MT873524 | ss(+) RNA | 14645 | 99.8 | RdRp, Helianthus annuus alphaendornavirus |
| Helianthus annuus leaf-associated mitovirus 1 | 208,030 | 130 | HlaMV1 | MT860450 | ss(+) RNA | 2280 | 43.9 | RdRp, Ophiostoma_mitovirus_3a |
| Helianthus annuus leaf-associated mitovirus 2 | 136 | 8446 | HlaMV2 | MT860451 | ss(+) RNA | 2192 | 36.2 | RdRp, Cryphonectria_parasitica_mitovirus_1-NB631 |
| Helianthus annuus leaf-associated partitivirus 1 | 5528 | 6816 | HlaPV1 | MT873525 | dsRNA | 1775 | 66.8 | RdRp, Raphanus sativus cryptic virus 1 |
| Helianthus annuus leaf-associated partitivirus 2 | 11,560 | 13,174 | HlaPV2 | MT873526 | dsRNA | 1593 | 60.5 | RdRp, Medicago sativa deltapartitivirus 1 |
| Helianthus annuus leaf-associated partitivirus 3 | 10,340 | 8 | HlaPV3 | MT873527 | dsRNA | 1721 | 76 | RdRp, Verticillium dahlia Partitivirus 1 |
| Helianthus annuus leaf-associated totivirus 1 | 12 | 3867 | HlaTV1 | MT873528 | dsRNA | 4924 | 50 | RdRp, Puccinia striiformis totivirus 4 |
| Helianthus annuus leaf-associated totivirus 2 | 4 | 1502 | HlaTV2 | MT873529 | dsRNA | 3854 | 37 | RdRp, Puccinia striiformis totivirus 2 |
| Helianthus annuus leaf-associated totivirus 3 | 0 | 540 | HlaTV3 | MT873530 | dsRNA | 2489 | 49 | RdRp, Puccinia striiformis totivirus 5 |
| Helianthus annuus leaf-associated totivirus 4 | 2 | 580 | HlaTV4 | MT873531 | dsRNA | 2887 | 79 | RdRp, Puccinia striiformis totivirus 5 |
| H_annus_leaf-associated_partitivirus_4 | 23 | 8 | HlaPV4 | MZ532542 | dsRNA | 381 | 67.5 | CP; Echinochloa crusgalli partitivirus |
| H_annus_leaf-associated_cryptic_virus_1 | 14,353 | 16,428 | HlaCV1 | MZ532543 | dsRNA | 1447 | 41.1 | CP; Pyrus pyrifolia cryptic virus |
| H_annus_leaf-associated_partitivirus_5 | 7020 | 3 | HlaPV5 | MZ532544 | dsRNA | 1625 | 67.6 | CP; Verticillium dahliae partitivirus 1 |
| H_annus_leaf-associated_totivirus_5 | 16 | 3083 | HlaTV5 | MZ532545 | dsRNA | 3121 | 39.8 | CP; Cronartium ribicola totivirus 3 |
| H_annus_leaf-associated_totivirus_6 | 0 | 1065 | HlaTV6 | MZ532546 | dsRNA | 2907 | 75.6 | CP; Uromyces totivirus B |
| H_annus_leaf-associated_totivirus_7 | 23 | 1468 | HlaTV7 | MZ532547 | dsRNA | 1965 | 39.3 | CP; Totiviridae sp. |
| H_annus_leaf-associated_totivirus_8 | 18 | 1826 | HlaTV8 | MZ532548 | dsRNA | 2061 | 62.5 | CP; Puccinia striiformis totivirus 3 |
| H_annus_leaf-associated_totivirus_9 | 6 | 719 | HlaTV9 | MZ532549 | dsRNA | 2536 | 36.6 | CP; Puccinia striiformis totivirus 1 |
| H_annus_leaf-associated_totivirus_10 | 8 | 873 | HlaTV10 | MZ532550 | dsRNA | 2380 | 65.2 | CP; Puccinia striiformis totivirus 1 |
| H_annus_leaf-associated_totivirus_11 | 9 | 417 | HlaTV11 | MZ532551 | dsRNA | 2189 | 45.9 | CP; Cronartium ribicola totivirus 5 |
| H_annus_leaf-associated_victorivirus_1 | 0 | 203 | HlaVV1 | MZ532552 | dsRNA | 1263 | 41.3 | CP; Alternaria alternata victorivirus 1 |
| H_annus_leaf-associated_totivirus_12 | 4 | 671 | HlaTV12 | MZ532553 | dsRNA | 1709 | 40.6 | CP; Totiviridae sp. |
| H_annus_leaf-associated_totivirus_13 | 18 | 386 | HlaTV13 | MZ532554 | dsRNA | 1613 | 83.6 | CP; Uromyces totivirus D |
| Bean yellow mosaic virus | 13 | 14 | HlaPoV1 | MZ532555 | ssRNA | 555 | 99.5 | CP; Bean yellow mosaic virus |
| H_annus_leaf-associated_cryptic_virus_2 | 1217 | 812 | HlaCV2 | MZ532556 | dsRNA | 1585 | 46.1 | CP; Raphanus sativus cryptic virus 1 |
| Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1-like virus | 2 | 4 | SsHADV-1 like | N/A | ssDNA | 197 | 96 | CP, Sclerotinia scleoritorum hypovirulence-associated DNA virus 1 |