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. 2021 Aug 27;9(9):957. doi: 10.3390/vaccines9090957

Figure 4.

Figure 4

RBD-Fc antigen with L-pampo induces cell-mediated responses. (A,B) BALB/c mice (total n = 42; n = 6/antigen-only; n = 8/Alum, AddaVax, AddaS03, or L-pampo (L); n = 4/CpG–Alum) were immunized with RBD-Fc with or without adjuvants i.m. on day 0 and day 21 as described in Figure 3A. On day 35, splenocytes were stimulated with the PepMixTM SARS-CoV-2(S-RBD) peptide pool, and RBD-specific IFN-γ secreting cells were analyzed as SFCs by ELISPOT assay (A) or total IFN-γ production by ELISA assay (B). Data shown are median ± IQR. Each dot represents an individual mouse. Data reflect 2 independent experiments (n = 3/antigen-only group; n = 4/Alum, AddaVax, AddaS03, or L-pampo (L) group; n = 2/CpG–Alum group in single experiment). Asterisks indicate statistically significant differences in comparison to the L-pampo (L) group. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; one way ANOVA with the Tukey’s test.