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(A) Sequence alignment of BATV and BUNV S segments and the primer’s respective placement for unique amplification. Visualisation with Geneious Prime 2021.0.3. (B,C) Representative agarose gel runs after amplifying BATV and BUNV L-, M- and S-segments using segment-specific primer sets (Supplementary Table S1). (B) BATV primer sets; (C) BUNV primer sets.
