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. 2021 Sep 18;13(9):1866. doi: 10.3390/v13091866

Figure 1.

Figure 1

The assembly of the full-length PEDV cDNA clone and recovery of rPEDV. (A) The schematic diagram for the assembly of the full-length cDNA clone of PEDV YN150. The full-length genome of the PEDV YN150 was divided into six contiguous cDNAs (F1–6). Restriction sites linking fragments are noted. (B) Wild-type PEDV, rPEDV, or mock-infected Vero cells were subjected to IFA using anti-PEDV S mAb 4B2 at 20 hpi (magnification, ×100). (C) Five genetic markers were verified by sequencing rPEDV genome. Four genetic markers at nucleotide positions 376, 787, 2842, and 11,564/11,566 are located on the ORF1ab, while one genetic marker at nucleotide position 23,882 is located on the Spike.