Figure 5.

The inhibitory effects of seven kinds of antioxidants on PEDV infection on Vero cells. Vero cells were incubated with seven antioxidants at various concentrations (2.5, 5, and 10 μM) for 1 h, and then infected with wild-type PEDV at a MOI of 0.01. (A) At 20 h post-infection, the titers of infected cell cultures were determined by TCID₅₀. NAC (50, 100, and 200 μM) and DMSO were used as the positive and negative controls. Data are presented as means ±SDs from three independent experiments. (B) PEDV S protein expression (green) in infected cells was analyzed by IFA using anti-PEDV S mAb 4B2. Nuclei (blue) were stained with DAPI. The percentage of PEDV-infected cells was counted based on the IFA results (bottom). NAC (50 μM) and DMSO were used as the positive and negative controls, * p < 0.05.