Body weight loss after PR8 H1N1 challenge of mice vaccinated with MVA-based vaccines. Combined conserved non-HA antigen MVA vaccines resulted in weight loss similar to an HA MVA vaccine. Mice were vaccinated intramuscularly two times, days 0 and 18–22, and challenged intranasally on day 42. (A) HA was superior to other single non-HA antigens. (B) Combined antigen vaccines resulted in less than 7% weight loss. (C) Data for NP and M2 vaccines, alone and combined. (D) Data for NP and M2eTML vaccines, alone and combined. (E) Data for NP and METRC vaccines, alone and combined. (F) Data for NP and METRS vaccines, alone and combined.