Figure 1.

Experimental timeline schema. Female macaques (n = 8) were inoculated with DENV 1–2 months prior to breeding. Around gestational day 45, dams were either inoculated with ZIKV or saline. Blood was drawn daily to measure ZIKV plasma viral loads (*) for 10 days, then twice weekly until viremia cleared, then weekly until delivery by Cesarean section (C-section). Infants had blood drawn for a ZIKV plasma viral load within the first week of life and participated in weekly neurodevelopmental exams (Schneider Neonatal Assessment Protocol; SNAP) weekly (^) for the first month of life. Precise gestational days at inoculation and C-section are noted in Table 1, and average infant ages at developmental testing are noted in Supplementary Table S4.